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Video Tutorials

Views:

6/16/17

Phenion FT Skin Models tissue culture upon arrival

This video tutorial guides you through the first working steps after the skin models have arrived in your lab.

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Views:

5/16/18

Phenion FT Skin Models - Compartment separation

This video sequence shows separation of epidermis from dermis after enzymatic treatment. Detailed information you will find in our protocol collection.

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Protocols

Protocol 3D Skin Comet assay

Protocol 3D Skin Comet assay

Protocol Phenion FT Skin Model Instructions for use

Protocol Phenion FT Skin Model Instructions for use

Protocol Phenion FT Skin Model RNA isolation I

Protocol Phenion FT Skin Model RNA isolation I

Protocol Phenion FT Skin Model RNA isolation II

Protocol Phenion FT Skin Model RNA isolation II

Protocol Phenion FT Skin Model Separation of Epidermis from Dermis

Protocol Phenion FT Skin Model Separation of Epidermis from Dermis

Protocol Phenion Feeder Cell Supported Keratinocyte Culture

Protocol Phenion Feeder Cell Supported Keratinocyte Culture

Protocol Phenion FT Skin Model Histology - Paraffin sections

Protocol Phenion FT Skin Model Histology - Paraffin sections

Protocol Phenion FT Skin Model Histology - Immunofluorescent labelling

Protocol Phenion FT Skin Model Histology - Immunofluorescent labelling

Protocol Phenion FT Skin Model Histology - Frozen sections

Protocol Phenion FT Skin Model Histology - Frozen sections

References & Further Reading

  • Skin Model Publications
  • Genotox Publications
  • OS-REp Publications
  • Hemicornea Publications
  • Other Publications
Dieckmann et al., 2016
A three-dimensional skin equivalent reflecting some aspects of in vivo aged skin.
Alili et al., 2014
A drug-induced accelerated senescence (DIAS) is a possibility to study aging in time lapse.
Bätz et al., 2013.
Esterase activity in excised and reconstructed human skin--biotransformation of prednicarbate and the model dye fluorescein diacetate.
Jäckh et al., 2011.
Characterization of enzyme activities of Cytochrome P450 enzymes, Flavin-dependent monooxygenases, N-acetyltransferases and UDP-glucuronyltransferases in human reconstructed epidermis and full-thickness skin models.
Ackermann et al., 2010
The Phenion full-thickness skin model for percutaneous absorption testing.
Zöller et al., 2008
Evaluation of beneficial and adverse effects of glucocorticoids on a newly developed full-thickness skin model.
Mewes et al., 2007
Elastin expression in a newly developed full-thickness skin equivalent.
Lee et al., 2006
An in vivo analysis of MMC-induced DNA damage and its repair.
Pfuhler et al., 2014
The Cosmetics Europe strategy for animal-free genotoxicity testing: project status up-date.
Alépée et al., 2016 Assessment of cosmetic ingredients in the in vitro reconstructed human epidermis test method EpiSkin™ using HPLC/UPLC-spectrophotometry in the MTT-reduction assay.

Barroso et al., 2015
Use of HPLC/UPLC-spectrophotometry for detection of formazan in in vitro Reconstructed human Tissue (RhT)-based test methods employing the MTT-reduction assay to expand their applicability to strongly coloured test chemicals.
Zorn-Kruppa et al., 2014
Determining the depthDetermining the Depth of Injury in Bioengineered Tissue Models of Cornea and Conjunctiva for the Prediction of All Three Ocular GHS Categories.
Engelke et al., 2013
A human hemi-cornea model for eye irritation testing: quality control of production, reliability and predictive capacity.
Klaka et al., 2017
A novel organotypic 3D sweat gland model with physiological functionality

Russell, William M.S. / Burch, Rex L. (1959): The Principles of Humane Experimental Technique. London: Methuen