Primary Human Skin Cells

Post circumcision, juvenile foreskin tissue from male individuals was stored appropriately and processed within 24 h. Cells are defined as in passage 0 (P0) when derived directly from the skin tissue and grown the first time in monolayer cell culture flasks. After confluency, the cells where harvested and propagated in new cell culture flasks to generate passage 1 cells (P1-cells) until harvested and stored above liquid nitrogen, at -196 °C. We provide 1.0 Mio P1-cells (keratinocytes or fibroblast) per cryo-vial. 

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Open Slideshow Human primary fibroblasts in culture for Phenion skin model

Morphology of human primary fibroblasts.

Open Slideshow Human primary cell culture for Phenion skin model

Cell culture flasks.

Open Slideshow Human primary keratinocytes in culture for Phenion skin model

Human primary keratinocytes in monolayer culture format.

Open Slideshow Feeder cells for co-culture of keratinocytes for Phenion skin model

Feeder cells support cultivation of Human Primary Keratinocytes.

For an optimal culture of Phenion keratinocytes we recommend to make use of feeder cells in a ratio of 1:1. Respective feeder cells, generated from dermal fibroblasts at higher passages, are growth-inhibited using Mitomycin C and routinely provided with 1.0 Mio cells per cryo-vial.

Beside other quality criteria, every cell batch is tested for the absence of HIV-1, Mycoplasma, Hepatitis B and C at an independent third party CRO. Respective certificates of analyses (CoA’s) are provided with every cell shipment (see examples). 

Views:

4/27/17

Primary Human Keratinocytes supported by Feeder Cells

The video shows a six days culture period.

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Views:

5/3/17

Primary Human Fibroplasts

The video shows a three days culture period.

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Certificate examples

Human primary keratinocytes in culture for Phenion skin model

CoA Phenion human dermal Fibroblasts

CoA Phenion human dermal Fibroblasts

Human primary cell culture for Phenion skin model

CoA Phenion human epidermal Keratinocytes

CoA Phenion human epidermal Keratinocytes

CoA Phenion human Feeder Cells

CoA Phenion human Feeder Cells

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