Post circumcision, juvenile foreskin tissue from male individuals was stored appropriately and processed within 24 h. Cells are defined as in passage 0 (P0) when derived directly from the skin tissue and grown the first time in monolayer cell culture flasks. After confluency, the cells where harvested and propagated in new cell culture flasks to generate passage 1 cells (P1-cells) until harvested and stored above liquid nitrogen, at -196 °C. We provide 1.0 Mio P1-cells (keratinocytes or fibroblast) per cryo-vial.
For an optimal culture of Phenion keratinocytes we recommend to make use of feeder cells in a ratio of 1:1. Respective feeder cells, generated from dermal fibroblasts at higher passages, are growth-inhibited using Mitomycin C and routinely provided with 1.0 Mio cells per cryo-vial.
Beside other quality criteria, every cell batch is tested for the absence of HIV-1, Mycoplasma, Hepatitis B and C at an independent third party CRO. Respective certificates of analyses (CoA’s) are provided with every cell shipment (see examples).
Discover further products
The human Phenion Full-Thickness Skin Models mimic native human skin in diverse dimensions, such as histological architecture and a wide spectrum of physiological skin parameters.
Phenion cell culture media are developed to serve the delicate nutrition needs for individual cell types and 3-dimensional complex skin tissues.
The innovative adapter module is designed to place the Phenion® Full-Thickness Skin Model precisely in the center of a Franz Cell.