Post circumcision, juvenile foreskin tissue from male individuals was stored appropriately and processed within 24 h. Cells are defined as in passage 0 (P0) when derived directly from the skin tissue and grown the first time in monolayer cell culture flasks. After confluency, the cells where harvested and propagated in new cell culture flasks to generate passage 1 cells (P1-cells) until harvested and stored above liquid nitrogen, at -196 °C. We provide 1.0 Mio P1-cells (keratinocytes or fibroblast) per cryo-vial.
For an optimal culture of Phenion keratinocytes we recommend to make use of feeder cells in a ratio of 1:1. Respective feeder cells, generated from dermal fibroblasts at higher passages, are growth-inhibited using Mitomycin C and routinely provided with 1.0 Mio cells per cryo-vial.
Beside other quality criteria, every cell batch is tested for the absence of HIV-1, Mycoplasma, Hepatitis B and C at an independent third party CRO. Respective certificates of analyses (CoA’s) are provided with every cell shipment (see examples).